Analysis Note

ControlPOSITIVE CONTROL: placenta, lung, and leukocytes.

Application

Western blot: 1:1,000. Reacts with a 79 kDa band
Immunoprecipitation: 5 µL/mL lysate
ELISA: 1:1000 – 1:100,000
Optimal working dilutions must be determined by the end user.

Anti-5′-Lipoxygenase Antibody detects level of 5′-Lipoxygenase & has been published & validated for use in ELISA, IP & WB.

General description

5′-Lipoxygenase is a critical enzyme in the formation of leukotrienes from arachidonic acid. Upon activation, the enzyme translocates to either a nuclear or plasma membrane compartment.

Immunogen

Purified human 5′-Lipoxygenase

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin in PBS with 50% Glycerol and 0.02% sodium azide

Format: Purified

Specificity

5′-Lipoxygenase